af860 rabbit polyclonal anti bid cell signaling  (Cell Signaling Technology Inc)

Journal: Helicobacter

Article Title: H. pylori -induced apoptosis in human gastric cancer cells mediated via the release of AIF from mitochondria

doi: 10.1111/j.1523-5378.2008.00646.x

Figure Lengend Snippet: Immunohistochemical staining of gastric tissue infected with H. pylori. Bright field images of gastric tissue infected with H. pylori (B-D) vs. control (A). The tissue was stained with H & E stain, arrows show healthy (A), AIF cytoplasmic staining (B) or apoptotic cells (C, D). Cells were immunostained with primary AIF-specific antibody, (AIF goat polyclonal, clone D-20; sc-9416; Santa Cruz Biotechnology) and donkey anti-goat horseradish peroxidase conjugated secondary antibody for DAB staining. Adequate antibody penetration and cell architecture preservation is evidenced by cytoplasmic staining.

Article Snippet: Proteins were electrotransferred onto polyvinylidine (Immobilon-P, Millipore, Bedford, MA) membranes blocked with 10% non-fat dry milk in TBS at 37oC, and incubated for 4 h at room temp or overnight at 4°C in TBS containing one of the following antibodies: (a) β-actin mouse monoclonal (Sigma) 1:4,000 dilution; (b) PARP rabbit polyclonal (Santa Cruz Biotech., Santa Cruz, CA) 1:250 dilution; (c) AIF goat polyclonal clone D-20 (sc-9416; Santa Cruz Biotechnology), 1:400 dilution; (d) Bid rabbit polyclonal (#2002; Cell Signalling), 1:500 dilution; (e) PARP mouse monoclonal (sc-8007; Santa Cruz Biotechnology), 1:500 dilution; (f) caspase 6 rabbit polyclonal (#9762; Cell Signaling), 1:500 dilution; (g) caspase 8 mouse monoclonal (#9746; Cell Signalling), 1:500 dilution; (h) lamin B goat polyclonal (Santa Cruz), 1:100 dilution in TBS (Tris buffered saline) containing 1% bovine serum album); or (i) β-catenin mouse monoclonal (Transduction Lab), 1:400 dilution.

Techniques: Immunohistochemical staining, Staining, Infection, Control, Preserving

Journal: Helicobacter

Article Title: H. pylori -induced apoptosis in human gastric cancer cells mediated via the release of AIF from mitochondria

doi: 10.1111/j.1523-5378.2008.00646.x

Figure Lengend Snippet: Immunofluorescent analysis of AIF. (A) DAPI-staining to visualize nucleus. (B) Immunohistochemical staining with rabbit polyclonal AIF primary antibody (sc-9417) followed by FITC-conjugated donkey anti-goat secondary antibody. (C) Merged image for viewing colocalization of AIF with DAPI-stained cytoplasmic and nuclear periphery staining. Bar =50 μm.

Article Snippet: Proteins were electrotransferred onto polyvinylidine (Immobilon-P, Millipore, Bedford, MA) membranes blocked with 10% non-fat dry milk in TBS at 37oC, and incubated for 4 h at room temp or overnight at 4°C in TBS containing one of the following antibodies: (a) β-actin mouse monoclonal (Sigma) 1:4,000 dilution; (b) PARP rabbit polyclonal (Santa Cruz Biotech., Santa Cruz, CA) 1:250 dilution; (c) AIF goat polyclonal clone D-20 (sc-9416; Santa Cruz Biotechnology), 1:400 dilution; (d) Bid rabbit polyclonal (#2002; Cell Signalling), 1:500 dilution; (e) PARP mouse monoclonal (sc-8007; Santa Cruz Biotechnology), 1:500 dilution; (f) caspase 6 rabbit polyclonal (#9762; Cell Signaling), 1:500 dilution; (g) caspase 8 mouse monoclonal (#9746; Cell Signalling), 1:500 dilution; (h) lamin B goat polyclonal (Santa Cruz), 1:100 dilution in TBS (Tris buffered saline) containing 1% bovine serum album); or (i) β-catenin mouse monoclonal (Transduction Lab), 1:400 dilution.

Techniques: Staining, Immunohistochemical staining